Identifying The Unknown Microorganism Given By The Instructor

Unknown #2 Lab Report Danielle Gudino BIO 211L Section 6 11/20/2014 Introduction This experiment was about identifying the unknown microorganism given by the instructor. This exercise is important to understand and apply all previous laboratory practices, as well as those learned in our first unknown exercise, for identifying the given unknown organism. With the knowledge and practice of performing biochemical and physiological identifying tests, I was able to determine the unknown bacteria. For this experiment, I was given Unknown #12 and used a series of tests to determine the specimen, further explained in this report. Materials and Methods For the Gram stain, I used a microscope slide, wash bottle of water, clothespin, and the reagents crystal violet, Gram’s iodine, ethyl alcohol, and safranin to observe whether the organism was Gram negative or positve. The method was placing and heat fixing a loopful of the unknown organism on the slide. The organisms were then stained with crystal violet for a minute, rinsed off, flooded with iodine for a minute, rinsed off, decolorized with alcohol for 30 seconds, and then finally stained with the counter-stain, safranin, for a minute. I streaked the unknown onto a TSA plate and incubated at 35 C. With a pure colony, we performed a second gram stain procedure and inoculated it into a TSA slant. I used BCP Lactose broth and a loop to perform a BCP Lactose test. The broth was inoculated with a loopful of the unknown and incubatedShow MoreRelatedHow to Write a Lab Report in Microbiology1637 Words   |  7 PagesHOW TO WRITE AN UNKNOWN LAB REPORT IN MICROBIOLOGY GENERAL Unknown reports in microbiology are written in scientific format. Scientific writing is written differently from other types of writing. The results of the exercise or experiment are what are being showcased, not the writing. The purpose of scientific writing is not to entertain, but to inform. 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